| 產品名稱 |
NCI-H1184 [H1184] |
| 商品貨號 |
B165253 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Cell Type |
lymphoblast |
| Product Format |
frozen |
| Culture Properties |
suspension, The cells grow as floating aggregates of round cell clusters. |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage L,carcinoma; small cell lung cancer |
| Age |
42 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
The line was established in September 1985. |
| Karyotype |
del(p13-p21) |
| Derivation |
The line was established in September 1985. |
| Clinical Data |
The patient was a smoker. A lymphoblastoid line from the same patient is available as ATCC CRL-5949. male Caucasian 42 years |
| Comments |
The line was established in September 1985. Fast growing. The patient was a smoker. 75 pack years. A lymphoblastoid line from the same patient is available as ATCC CRL-5949. |
| Complete Growth Medium |
The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%. |
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation with subsequent resuspension in fresh medium. The cells are slow to grow and the clusters will get larger over time. Some clusters can lightly adhere to the flask. These cells cannot be counted accurately, if the cell clusters are dispersed into single cells, they will lose viability.
Add fresh medium every 2 to 3 days. |
| Cryopreservation |
RPMI 1640 medium 85%; fetal bovine serum, 10%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1985 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
