| 產品名稱 | NCI-H1299 |
|---|---|
| 商品貨號 | B165256 |
| Organism | Homo sapiens, human |
| Tissue | lung; derived from metastatic site: lymph node |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | carcinoma; non-small cell lung cancer |
| Age | 43 years adult |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The cell line was established from a lymph node metastasis of the lung from a patient who had received prior radiation therapy. |
| Clinical Data | male 43 years adult Caucasian |
| Genes Expressed | These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein.
neuromedin B,The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B. |
| Cellular Products | neuromedin B |
| Comments | The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. They reported to be able to synthesize the peptide neuromedin B (NMB) at 0.1 pmol/mg protein, but not the gastrin releasing peptide (GRP). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin:X CSF1PO:12 D13S317:12 D16S539:12,13 D5S818:11 D7S820:10 THO1:6,9.3 TPOX:8 vWA: 16,17,18 |
| Name of Depositor | AF Gazdar, JD Minna |
| Deposited As | Homo sapiens |
| References | Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed: 8663350 This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
