| 產品名稱 |
NCI-H1341 [H1341] |
| 商品貨號 |
B165257 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; Derived from metastatic site: cervix |
| Product Format |
frozen |
| Morphology |
floating aggregates of round cells with a few attached cells. |
| Culture Properties |
suspension |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
carcinoma; small cell lung cancer |
| Gender |
female |
| Applications |
The line was established in February 1986. |
| Derivation |
The line was established in February 1986. |
| Clinical Data |
The tissue donor was a non-smoker. female |
| Comments |
The line was established in February 1986. The tissue donor was a non-smoker. |
| Complete Growth Medium |
ACL-4 medium (serum-free)
The base medium for this cell line is ATCC formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:- 0.02 mg/ml insulin
- 0.01 mg/ml transferrin
- 25 nM sodium selenite (final conc.)
- 50 nM Hydrocortisone (final conc.)
- 1 ng/ml Epidermal Growth Factor (do not filter)
- 0.01 mM ethanolamine (final conc.)
- 0.01 mM phosphorylethanolamine (final conc.)
- 100 pM triiodothyronine (final conc.)
- 0.5% (w/v) bovine serum albumin (final conc.)
- 10 mM HEPES
- 0.5 mM sodium pyruvate (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5mM)
|
| Subculturing |
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) Cultures can be maintained by the addition of fresh medium. Cells grow as floating aggregates of round cells with a few attached cells. |
| Cryopreservation |
Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1986 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
