| 產(chǎn)品名稱 |
NCI-H1581 [H1581] |
| 商品貨號 |
B165270 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Cell Type |
large cell |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
mixed, adherent and suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage 4, non-small cell lung cancer |
| Age |
44 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was established in January 1987. |
| Clinical Data |
44 years
Caucasian
male
The patient was a smoker.
75 pack years. |
| Complete Growth Medium |
The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%. |
| Subculturing |
Cultures can be maintained by the addition of fresh medium. Cells grow mostly as floating aggregates of round cell clusters with some attached cells. Clusters are alive while single cells have very low viability. Attached cells can be shaken loose. |
| Cryopreservation |
Freeze medium: culture medium, 85%; FBS, 10%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1987 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
