| 產品名稱 |
NCI-H1618 [H1618] |
| 商品貨號 |
B165271 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; derived from metastatic site: bone marrow |
| Product Format |
frozen |
| Culture Properties |
suspension, multicell aggregates |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage E,carcinoma; small cell lung cancer |
| Age |
55 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was established in April 1987. |
| Clinical Data |
Caucasian
female
The patient was a smoker.
50 pack years. |
| Complete Growth Medium |
HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
|
| Subculturing |
Cells can be maintained by the addition of fresh medium
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 9 D16S539: 11,13 D5S818: 10 D7S820: 8 THO1: 6 TPOX: 8,11 vWA: 16,17 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
April, 1987 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
|
