| 產品名稱 | NCI-H1770 [H1770] |
|---|---|
| 商品貨號 | B165284 |
| Organism | Homo sapiens, human |
| Tissue | lung; derived from metastatic site: lymph node |
| Cell Type | neuroendocrine |
| Product Format | frozen |
| Culture Properties | suspension, multicell aggregates |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | stage 4, carcinoma; non-small cell lung cancer |
| Age | 57 years |
| Gender | male |
| Ethnicity | Caucasian |
| Images |  |
| Derivation | The line was established in November 1987. |
| Clinical Data | 57 years
Caucasian, White
male
The tissue donor was a non-smoker. |
| Comments | A lymphoblastoid line from the same patient is available as ATCC CRL-5960. |
| Complete Growth Medium | The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%. |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.
Cells grow as floating aggregates of round cell clusters.
Medium Renewal: Add fresh medium as cell density increases. |
| Cryopreservation | Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 11,13 D13S317: 13 D16S539: 12,13 D5S818: 11,12 D7S820: 11 THO1: 9 TPOX: 11 vWA: 18,19 |
| Name of Depositor | AF Gazdar, JD Minna |
| Deposited As | Homo sapiens |
| Year of Origin | 1987 |
| References | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
