| 產品名稱 |
NCI-H1836 [H1836] |
| 商品貨號 |
B165289 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Product Format |
frozen |
| Culture Properties |
floating aggregates of round cell clusters |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage L, carcinoma; classic small cell lung cancer |
| Age |
52 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Karyotype |
del(p11-qter) |
| Derivation |
The line was established in February 1988.
The patient was a smoker who received prior chemotherapy and radiation therapy. |
| Clinical Data |
male
Caucasian
52 years |
| Complete Growth Medium |
HITES medium supplemented with 5% fetal bovine serum
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc.)
|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation with subsequent resuspension in fresh medium. Medium Renewal: Add fresh medium as cell density increases. |
| Cryopreservation |
culture medium, 90%; additional fetal bovine serum, 5%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 10,12 D16S539: 11,13 D5S818: 11 D7S820: 11,12 THO1: 8,9.3 TPOX: 9,10 vWA: 16 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1988 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
