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NCI-H1882 [H1882]

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編號(hào):B165294

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產(chǎn)品名稱	NCI-H1882 [H1882]
商品貨號(hào)	B165294
Organism	Homo sapiens, human
Tissue	lung; Derived from metastatic site: bone marrow
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	stage E,carcinoma; small cell lung cancer
Age	59 years
Gender	male
Ethnicity	Caucasian
Applications	The line was established in April 1988.
Images
Derivation	The line was established in April 1988.
Clinical Data	The patient was a smoker. male Caucasian 59 years
Comments	The line was established in April 1988. The patient was a smoker. 40 pack years.
Complete Growth Medium	HITES medium supplemented with 5% fetal bovine serum The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium 0.005 mg/ml Insulin 0.01 mg/ml Transferrin 30nM Sodium selenite (final conc.) 10 nM Hydrocortisone (final conc.) 10 nM beta-estradiol (final conc.) extra 2mM L-glutamine (for final conc. of 4.5 mM) 5% fetal bovine serum (final conc.)
Subculturing	Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 twice weekly is recommended. Medium renewal: Every 2 to 3 days
Cryopreservation	RPMI 1640, 85% ; FBS, 10%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C
STR Profile	Amelogenin: X,Y CSF1PO: 11 D13S317: 12 D16S539: 12 D5S818: 11 D7S820: 10,12 THO1: 6 TPOX: 8,9 vWA: 17
Name of Depositor	AF Gazdar, JD Minna
Deposited As	Homo sapiens
Year of Origin	1988
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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