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NCI-H1930 [H1930]

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編號:B165296

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產品名稱	NCI-H1930 [H1930]
商品貨號	B165296
Organism	Homo sapiens, human
Tissue	lung
Product Format	frozen
Culture Properties	suspension, multicell aggregates
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	stage L, carcinoma; classic small cell lung cancer
Age	41 years
Gender	male
Ethnicity	White
Storage Conditions	liquid nitrogen vapor phase
Karyotype	del(p13-p21)
Derivation	The line was established in May 1988 from lymph node, a metastatic site.
Clinical Data	The tissue donor was a non-smoker.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cells grow as floating aggregates of round cell clusters. Clusters are alive while single cells have very low viability. Do not try to break up the clusters as this would decrease the viability. Centrifuge cell suspension every 2 to 3 days. After centrifugation, aspirate supernatant down to 1 mL above cell pellet. Resuspend cells by gentle swirling in 3 to 4 mL of fresh medium. Seed at a concentration of 2 to 2.5 x 10⁴ viable cells/mL. Transfer to new culture flasks when necessary.
Cryopreservation	50% FBS + 40% Culture Media + 10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
Name of Depositor	AF Gazdar, JD Minna
Deposited As	Homo sapiens
Year of Origin	1988
References	NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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