| 產品名稱 |
NCI-H220 [H220] |
| 商品貨號 |
B165325 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Product Format |
frozen |
| Culture Properties |
suspension, floating aggregates of round cells with some attached cells |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage E,carcinoma; classic small cell lung cancer |
| Age |
51 years |
| Gender |
male |
| Ethnicity |
Black |
| Applications |
The line was established in February 1980. |
| Derivation |
The line was established in February 1980. |
| Clinical Data |
The tissue donor was a non-smoker. male Black 51 years |
| Comments |
The line was established in February 1980. The tissue donor was a non-smoker. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 3 to 5 days Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| STR Profile |
Amelogenin: X,Y
CSF1PO: 10
D13S317: 12
D16S539: 10
D5S818: 11
D7S820: 10, 11
TH01: 7,9.3
TPOX: 8,11
vWA: 16,18 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| Year of Origin |
1980 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
