| 產品名稱 |
NCI-H498 [H498] |
| 商品貨號 |
B165346 |
| Organism |
Homo sapiens, human |
| Tissue |
cecum |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
suspension, with some loosely adherent cells |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
colorectal adenocarcinoma |
| Age |
56 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy. The cells form glandular structures and secrete mucin. The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA. Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 51; range = 47 to 54.
This is a hyperdiploid human cell line. The modal chromosome number is 51, occurring in 42% of the cells. The rate of higher ploidies is 5%. The 2q+ is the only marker chromosome detected and common to all cells. There are four copies for N7; 3 copies each for N8, N13 and N22, and a single copy for the X. |
| Derivation |
This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy. Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity. |
| Clinical Data |
This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy. Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity. male Caucasian 56 years |
| Antigen Expression |
Rh+; CA19-9 antigen; TAG-72 antigen |
| Genes Expressed |
This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. carcinoembryonic antigen (CEA), 826 ng/ml per 10 exp6 cells per 10 days; mucin,Rh+; CA19-9 antigen; TAG-72 antigen,The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA. |
| Cellular Products |
carcinoembryonic antigen (CEA), 826 ng/ml per 10 exp6 cells per 10 days; mucin |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments |
This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy. The cells form glandular structures and secrete mucin. The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA. Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium.
Alternatively, the cells may be collected by centrifugation and then resuspended at 2 to 4 X 10(4) viable cells/ml. When the cell concentration becomes heavy with large clumps, aspirate well and and dilute approximately 1:10.
If the clumps are allowed to get too large, most of the cells will die. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 9,11 D16S539: 9,11 D5S818: 13 D7S820: 8,11 THO1: 7 TPOX: 8 vWA: 15 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor |
AF Gazdar |
| Deposited As |
Homo sapiens |
| References |
Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345
Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells
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