| 產(chǎn)品名稱(chēng) |
NCI-H735 [H735] |
| 商品貨號(hào) |
B165364 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; derived from metastatic site: liver |
| Product Format |
frozen |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
stage E, carcinoma; small cell lung cancer |
| Age |
58 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The line was established in August 1984. |
| Clinical Data |
The patient received prior chemotherapy. The patient was a smoker. female Caucasian 58 years |
| Complete Growth Medium |
HITES medium (serum free) HITES medium is formulated at the ATCC as follows: Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006) Insulin 0.005 mg/ml Transferrin 0.01 mg/ml Sodium selenite 30 nM Hydrocortisone 10 nM beta-estradiol 10 nM HEPES 10 mM L-glutamine 2 mM (in addition to that in the base medium)
|
| Cryopreservation |
Freeze Medium: Complete growth medium supplemented with 5% FBS and 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: 5% CO2 in air. |
| STR Profile |
Amelogenin: X CSF1PO: 12,14 D13S317: 11,14 D16S539: 14 D5S818: 9,11 D7S820: 8,11 THO1: 8,9.3 TPOX: 11 vWA: 18,19 |
| Year of Origin |
1984 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
