| Subculturing |
CRL-5840 cells grow as aggregates in suspension.
Cultures may lose viability when the aggregates are dispersed.
Cultures can be maintained by the addition of fresh medium. A split ratio of 1:2 to 1:4 should be used and the cell line is subcultured when there are numerous healthy appearing clusters present in suspension.
Medium Renewal: Every 2 to 3 days
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| STR Profile |
Amelogenin: X,Y CSF1PO: 9,12 D13S317: 11,14 D16S539: 12 D5S818: 12 D7S820: 9,10 THO1: 6,9 TPOX: 8,10 vWA: 15,16 |
| References |
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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