| Applications |
The NFS-25 C-3 cell line was derived from a spontaneous lymphoma. They are negative for ThB, surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox). |
| Comments |
The NFS-25 C-3 cell line was derived from a spontaneous lymphoma. The cells bear markers consistent with identification as large pre-B lymphoblasts [(Ly 17, CD32), (Ly 1, CD5), (Lyb 2, Ly 5(B220), CD45R)]. They are negative for ThB, surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 10 mM HEPES, 3 mM glutamine, 1 mM oxaloacetic acid, 0.2 units/ml insulin, 0.5 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol, 2 mcg/ml transferrin, 80%; NCTC medium 109, 10%; fetal bovine serum, 10%
|
| References |
Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
|
