| 產品名稱 |
NFS-5 C-1 |
| 商品貨號 |
B165393 |
| Organism |
Mus musculus, mouse |
| Cell Type |
pre-B lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
NFS/N |
| Applications |
The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus. They are negative for ThB, surface immunoglobulin (sIg) and Ia. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus. |
| Antigen Expression |
CD5 +; CD32 +; CD45R + |
| Genes Expressed |
ecotropic retrovirus; mink cell focus forming retrovirus,CD5 +; CD32 +; CD45R + |
| Cellular Products |
ecotropic retrovirus; mink cell focus forming retrovirus |
| Comments |
The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus. The cells bear a pattern of markers consistent with identification as large pre-B lymphoblasts (Ly 17, CD32. Ly 1, CD5. Lyb 2, Ly 5(B220), CD45R). They are negative for ThB, surface immunoglobulin (sIg) and Ia. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Modified Dulbecco's medium with 0.002 mg/ml transferrin and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
WJ Davidson, HC Morse |
| Deposited As |
Mus musculus |
| References |
Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201
|
