| 產品名稱 | NL20 |
|---|---|
| 商品貨號 | B165399 |
| Organism | Homo sapiens, human |
| Tissue | lung/bronchus |
| Cell Type | Epithelial |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain SV40 viral sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Accident victim |
| Age | 20 years |
| Gender | female |
| Ethnicity | Caucasian, White |
| Applications | The non-tumorigenic NL20 cell line and the tumorigenic NL20-TA cell line form a pair of immortal cell lines that can be used to study tumor progression. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | near diploid |
| Images |  |
| Derivation | NL20 (ATCC CRL-2503) is an immortalized, nontumorigenic human bronchial epithelial cell line derived from normal bronchus taken from an accident victim at autopsy.
The cell line was established by transfection with the origin of replication-defective SV40 large T plasmid, p129.
After three months the tumor was removed and placed in culture. At passage 3, these cells were re-injected into nude mice.
NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line. |
| Clinical Data | 20 years Caucasian, White female |
| Tumorigenic | No |
| Effects | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments | NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line. After three months the tumor was removed and placed in culture. At passage 3, these cells were re-injected into nude mice. One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. This cell line (ATCC CRL-2504) remains tumorigenic up to at least passage 250. |
| Complete Growth Medium | Ham's F12 medium with 1.5 g/L sodium bicarbonate, 2.7 g/L glucose, 2.0 mM L-glutamine, 0.1 mM nonessential amino acids, 0.005 mg/ml insulin, 10 ng/ml epidermal growth factor, 0.001 mg/ml transferrin, 500 ng/ml hydrocortisone and 4% fetal bovine serum
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:12 to 1:20 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
|
| Cryopreservation | Culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37.0°C |
| Name of Depositor | JH Schiller |
| Deposited As | human |
| Passage History | One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. This cell line (ATCC CRL-2504) remains tumorigenic up to at least passage 250. |
| References | Schiller JH, et al. Phenotypic, molecular and genetic characterization of transformed human bronchial epithelial cell strains. Int. J. Oncol. 4: 461-470, 1994. Schiller JH, Bittner G. Loss of the tumorigenic phenotype with in vitro, but not in vivo, passaging of a novel series of human bronchial epithelial cell lines: possible role of an alpha 5/beta 1-integrin-fibronectin interaction. Cancer Res. : 6215-6221, 1995. PubMed: 8521416 Wittenkeller JL, et al. Comparison of spontaneous and induced mutation rates in an immortalized human bronchial epithelial cell line and its tumorigenic derivative. Oncology 54: 335-341, 1997. PubMed: 9216860 Schiller JH, et al. Karyotypic changes associated with spontaneous acquisition and loss of tumorigenicity in a human transformed bronchial epithelial cell line: evidence for in vivo selection of transformed clones. In Vitro Cell. Dev. Biol. Anim. 34: 283-289, 1998. PubMed: 9590501 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
