| 產(chǎn)品名稱 |
PA317 LXSN |
| 商品貨號 |
B165470 |
| Organism |
Mus musculus, mouse |
| Tissue |
embryo |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
2 Cells contain human papilloma viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Age |
embryo |
| Strain |
NIH/Swiss |
| Applications |
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418. PA317 LXSN cells produce an amphoteric retrovirus with an empty neo control vector, 5' long terminal repeat (LTR) from the Moloney murine leukemia virus (MoMuLV) multiple cloning region and 3' LTR from MoMuLV. The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter. This line is useful as a negative control for the use of PA317 LXSN 16E6E7 (see ATCC CRL-2203). |
| Derivation |
PA317 LXSN is a packaging cell line developed by transfection of the retrovirus vector pLXSN into the Psi-2 ecotropic packaging cell line. Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418. |
| Comments |
PA317 LXSN is a packaging cell line developed by transfection of the retrovirus vector pLXSN into the Psi-2 ecotropic packaging cell line. Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418. PA317 LXSN cells produce an amphoteric retrovirus with an empty neo control vector, 5' long terminal repeat (LTR) from the Moloney murine leukemia virus (MoMuLV) multiple cloning region and 3' LTR from MoMuLV. The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter. This line is useful as a negative control for the use of PA317 LXSN 16E6E7 (see ATCC CRL-2203). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Name of Depositor |
DA Galloway |
| Deposited As |
Mus musculus |
| References |
Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902
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