| 產(chǎn)品名稱 | PC-12 |
|---|---|
| 商品貨號 | B165487 |
| Organism | Rattus norvegicus, rat |
| Tissue | adrenal gland |
| Product Format | frozen |
| Morphology | small irregularly shaped cells |
| Culture Properties | floating clusters; few scattered lightly attached cells. |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | pheochromocytoma |
| Gender | male |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | 40 chromosomes; 38 autosomes plus XY
Ref  Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 |
| Images |  |
| Derivation | The PC-12 cell line was derived from a transplantable rat pheochromocytoma. |
| Clinical Data | male |
| Receptor Expression | nerve growth factor (NGF), expressed |
| Genes Expressed | catecholamines; dopamine; norepinephrine |
| Cellular Products | catecholamines; dopamine; norepinephrine |
| Tumorigenic | Yes |
| Effects | Yes, in New England Deaconess Hospital strain rats |
| Comments | The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks. The cells do not synthesize epinephrine. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
|
| Subculturing | Protocol: Volumes used for this protocol are for a 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time | 48 hrs |
| Name of Depositor | B Patterson |
| Deposited As | Rattus sp. |
| References | Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317 Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712 Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125 U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005 Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 |
| Cross References | Nucleotide (GenBank) : AF311055 Rattus norvegicus thioredoxin mRNA, complete cds. Nucleotide (GenBank) : AF319950 Rattus norvegicus glutaredoxin mRNA, complete cds. Nucleotide (GenBank) : AF311054 Rattus norvegicus APEX (Apex) mRNA, partial cds, alternatively spliced. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
