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R1.1

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編號:B165549

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產(chǎn)品名稱	R1.1
商品貨號	B165549
Organism	Mus musculus, mouse
Tissue	thymus
Cell Type	T lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	lymphoma
Strain	C58/J
Applications	Established from a spontaneous thymoma. Sensitive to 0.001 mM cortisol and 10 mcg/ml PHA. Tested and found negative for ectromelia virus (mousepox).
Derivation	Established from a spontaneous thymoma.
Antigen Expression	H-2k; Thy-1.2; TL
Genes Expressed	H-2k; Thy-1.2; TL
Comments	Established from a spontaneous thymoma. Sensitive to 0.001 mM cortisol and 10 mcg/ml PHA. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor	P Ralph
Deposited As	Mus musculus
References	Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976. Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

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