| 產品名稱 |
RA3-2C2/1 |
| 商品貨號 |
B165567 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Applications |
The antibody reacts with an antigen on normal B lymphocytes, a fraction of sIg- bone marrow cells and most plasma cells. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused S194/5.XX0.BU.1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse B cell antigen (p50, 50000 dalton) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse B cell antigen (p50, 50000 dalton) |
| Comments |
Animals were immunized with the mouse cell line RAW 112. Spleen cells were fused S194/5.XX0.BU.1 myeloma cells. The antibody reacts with an antigen on normal B lymphocytes, a fraction of sIg- bone marrow cells and most plasma cells. It precipitates a 50000 dalton (p50) molecule from bone marrow, spleen, thymus and Abelson leukemia virus transformed cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
RL Coffman |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
Rotter V, et al. Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50. J. Virol. 36: 547-555, 1980. PubMed: 6159484
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