Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rat astrocyte; ependymal cell, retinal Muller cell and leptomeningeal cell (rat neural antigen-2, RAN-2)
Cellular Products
immunoglobulin; monoclonal antibody; against rat astrocyte; ependymal cell, retinal Muller cell and leptomeningeal cell (rat neural antigen-2, RAN-2)
Comments
Animals were immunized with enriched preparations of rat astrocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not react with cultured neurons, oligodendrocytes, Schwann cells, microglia or various non-neuronal cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
PF Bartlett, M Raff
Deposited As
mouse (B cell); mouse (myeloma)
References
Bartlett PF, et al. Rat neural antigen-2 (RAN-2): a cell surface antigen on astrocytes, ependymal cells, Muller cells and lepto-meninges defined by a monoclonal antibody. Brain Res. 204: 339-351, 1980. PubMed: 7006734
