| 產(chǎn)品名稱 |
RAW 309F.1.1 |
| 商品貨號 |
B165581 |
| Organism |
Mus musculus, mouse |
| Tissue |
spleen |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Applications |
The cells are negative for surface immunoglobulin (sIg-), Thy-1.2 (Thy-1.2-) and Ia (Ia-). The cells do not pinocytose neutral red, phagocytose zymosan or latex beads and do not secrete lysozyme. The line was derived from the RAW 309 tumor (see also ATCC TIB-69). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
The line was derived from the RAW 309 tumor (see also ATCC TIB-69). |
| Antigen Expression |
H-2d; H-2b |
| Genes Expressed |
Abelson murine leukemia virus (infectious),H-2d; H-2b |
| Cellular Products |
Abelson murine leukemia virus (infectious) |
| Comments |
The cells are negative for surface immunoglobulin (sIg-), Thy-1.2 (Thy-1.2-) and Ia (Ia-). The cells do not pinocytose neutral red, phagocytose zymosan or latex beads and do not secrete lysozyme. The line was derived from the RAW 309 tumor (see also ATCC TIB-69). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor |
WC Raschke |
| Deposited As |
Mus musculus |
| References |
Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
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