| 產品名稱 | RD |
|---|---|
| 商品貨號 | B165586 |
| Organism | Homo sapiens, human |
| Tissue | muscle |
| Product Format | frozen |
| Morphology | spindle cells and large multinucleated cells |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | rhabdomyosarcoma |
| Age | 7 years |
| Gender | female |
| Ethnicity | Caucasian |
| Applications | This cell line can be used for the detection of viruses. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | unstable within a hyperdiploid bimodal stemline number of 49 and 50. Twenty-two cells had chromosome associations, 15 cells microchromosomes, 2 cells had fragments, 2 cells with breaks, 2 cells with achromatic gaps and 1 cell with a secondary constriction Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Images |  |
| Clinical Data | 7 years Caucasian female |
| Genes Expressed | myoglobin; myosin ATPase |
| Cellular Products | myoglobin myosin ATPase |
| Virus Susceptibility | Human poliovirus 1
Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) Herpes simplex virus Vaccinia virus |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 10,11 D5S818: 11 D7S820: 8,12 THO1: 9.3 TPOX: 9 vWA: 18 |
| Isoenzymes | G6PD, B |
| Name of Depositor | RM McAllister |
| Deposited As | Homo sapiens |
| References | American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. McAllister RM, et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949 Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733 Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Hahon N, Cooke KO. Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. J. Virol. 1: 326, 1967. PubMed: 4918236 Guerin E, et al. Stromelysin-3 induction and interstitial collagenase repression by retinoic acid. J. Biol. Chem. 272: 11088-11095, 1997. PubMed: 9111003 Jeffers M, et al. Degradation of the Met tyrosine kinase receptor by the ubiquitin-proteasome pathway. Mol. Cell. Biol. 17: 799-808, 1997. PubMed: 9001234 Massuda ES, et al. Regulated expression of the diphtheria toxin A chain by a tumor-specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. Proc. Natl. Acad. Sci. USA 94: 14701-14706, 1997. PubMed: 9405676 Tiger CF, et al. Presence of laminin alpha5 chain and lack laminin alpha1 chain during human muscle development and in muscular dystrophies. J. Biol. Chem. 272: 28590-28595, 1997. PubMed: 9353324 Sabbioni S, et al. GOK: a gene at 11p15 involved in rhabdomysarcoma and rhabdoid tumor development. Cancer Res. 57: 4493-4497, 1997. PubMed: 9377559 Xiao GH, et al. The Tuberous Sclerosis 2Gene Product, Tuberin, Functions as a Rab5 GTPase Activating Protein (GAP) in Modulating Endocytosis. J. Biol. Chem. 272: 6097-6100, 1997. PubMed: 9045618 |
| Cross References | Nucleotide (GenBank) : U09279 Human type XIX collagen (COL19A1) mRNA, partial cds. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
