| 產(chǎn)品名稱 |
RPMI 1846 |
| 商品貨號 |
B165626 |
| Organism |
Mesocricetus auratus, hamster, Syrian golden |
| Tissue |
skin |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
melanotic melanoma |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 67; range = 61 to 69 |
| Genes Expressed |
melanin |
| Cellular Products |
melanin |
| Tumorigenic |
Yes |
| Effects |
Yes, in newborn hamsters |
| Virus Susceptibility |
Vesicular stomatitis virus
Human poliovirus 1
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
-
Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
-
Add 2.0 mL to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 mL to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
-
Incubate cultures at 37°C.
Subcultivation Ratio: 1: 2 to 1: 5
Medium Renewal: Two to three times weekly
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation |
Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions |
Temperature: 37°C
Atmosphere: 5% Carbon dioxide (CO2) |
| Name of Depositor |
GE Moore |
| Deposited As |
Mesocricetus auratus |
| References |
Moore GE, et al. Continuous culture of a melanotic cell line from the golden hamster. Science 137: 986-987, 1962. PubMed: 14475649
Mount D, et al. Culture of malignant tumors of the Syrian hamster. J. Natl. Cancer Inst. 31: 1217-1237, 1963. PubMed: 14071829
Hsu TC, Kellogg DS Jr.. Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens. J. Natl. Cancer Inst. 25: 221-235, 1960. PubMed: 14403619
Fortner JG, et al. Transplantable tumors of the Syrian (golden) hamster. I. Tumors of the alimentary tract, endocrine glands and melanomas. Cancer Res. 21: 161-198, 1961. PubMed: 13700922
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