| 產品名稱 |
S49.1G.3 |
| 商品貨號 |
B165650 |
| Organism |
Mus musculus, mouse |
| Cell Type |
T lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
BALB/c |
| Applications |
The cells are sensitive to 0.01 mM thymidine, 0.001 mM hydrocortisone and 5 mcg/ml phytohemagglutinin. Tested and found negative for ectromelia virus (mousepox). |
| Antigen Expression |
H-2d; Thy-1.2; TL |
| Genes Expressed |
H-2d; Thy-1.2; TL |
| Comments |
This variant of S49.1 (ATCC TIB-28) was selected for resistance to 1 mM 6-thioguanine. The cells are sensitive to 0.01 mM thymidine, 0.001 mM hydrocortisone and 5 mcg/ml phytohemagglutinin. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor |
A Harris |
| Deposited As |
Mus musculus |
| References |
Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
|
