| 產品名稱 | SK-N-AS |
|---|---|
| 商品貨號 | B165713 |
| Organism | Homo sapiens, human |
| Tissue | brain; derived from metastatic site: bone marrow |
| Cell Type | neuroblast |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | neuroblastoma |
| Age | 6 years |
| Gender | female |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | Bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma. |
| Clinical Data | 6 years Caucasian female |
| Receptor Expression | insulin-like growth factor I (IGF-I), expressed |
| Tumorigenic | Yes |
| Effects | Yes, forms tumors in nude mice |
| Comments | SK-N-AS cells synthesize Insulin-like growth factor II (IFG-II) and IGF-II RNA and possess type I IGF receptors. Ref   El-Badry OM, et al. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840 Retinoic acid partially inhibits proliferation, and cells fail to differentiate. RefGaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405 The cells exhibit low or no MDR1 expression. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:5 to 1:10 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation | Freeze Medium: Culture medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 10,12 D13S317: 9 D16S539: 14 D5S818: 11,12 D7S820: 11,13 THO1: 9.3 TPOX: 11,12 vWA: 16,17 |
| Population Doubling Time | 39 hours |
| Name of Depositor | C Helson |
| Deposited As | Homo sapiens |
| Year of Origin | 1981 |
| References | Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792 Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824 Gaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405 El-Badry OM, et al. Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840 bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
