| 產(chǎn)品名稱(chēng) |
SK-UT-1B |
| 商品貨號(hào) |
B165724 |
| Organism |
Homo sapiens, human |
| Tissue |
uterus; endometrium |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
grade III,mesodermal tumor (mixed); consistent with leiomyosarcoma |
| Age |
75 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Karyotype |
(P27) hypodiploid to diploid |
| Clinical Data |
75 years Caucasian female |
| Antigen Expression |
Blood type B; Rh+ |
| Genes Expressed |
Blood type B; Rh+ |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice |
| Comments |
This is a substrain of SK-UT-1 (see ATCC HTB-114) which reportedly differs in morphology, karyology and in the type of tumor which develops on animal inoculation.
The cells form well differentiated adenocarcinomas consistent with endometrial carcinoma. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X CSF1PO: 10,11 D13S317: 10,13 D16S539: 12,14 D5S818: 10,11 D7S820: 9,10 THO1: 7 TPOX: 8 vWA: 16 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1-2 PGM1, 1 PGM3, 1 |
| Name of Depositor |
G Trempe |
| Deposited As |
Homo sapiens |
| References |
Chen TR. SK-UT-1B, a human tumorigenic diploid cell line. Cancer Genet. Cytogenet. 33: 77-81, 1988. PubMed: 3383166
Chen TR. SK-UT-1B cell line has the diploid karyotype. A reply. Cancer Genet. Cytogenet. 48: 139-141, 1990. PubMed: 2372783
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
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