| 產(chǎn)品名稱 |
SM27-1045 |
| 商品貨號 |
B165730 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Product Format |
frozen |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: C57BL/6 (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with a unique cell marker (EM10) that is expressed on stem and progenitor cells, while being less accessible or absent on more mature cells. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against EM10 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against EM10 |
| Comments |
Animals were immunized with CD34 positive, CD38 negative human bone marrow cells. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody reacts with a unique cell marker (EM10) that is expressed on stem and progenitor cells, while being less accessible or absent on more mature cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Iscove's modified Dulbecco's medium, 75%; fetal bovine serum, 20%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM; kappa light chain |
| Name of Depositor |
SyStemix, Inc. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Hill BL, et al. Methods of obtaining compositions enriched for hematopoietic stem cells, antibodies for use therein, compositions derived therefrom and methods of use thereof. US Patent 5,814,440 dated Sep 29 1998
|
