| 產品名稱 |
SW1417 [SW-1417] |
| 商品貨號 |
B165793 |
| Organism |
Homo sapiens, human |
| Tissue |
colon |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Dukes' type C, grade III, colorectal adenocarcinoma |
| Age |
53 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
female 53 years Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity. Caucasian |
| Antigen Expression |
blood type B; Rh+ |
| Oncogene |
myc +; ras +; fos +; sis +; p53 -; myb -; abl -; ros -; src - |
| Genes Expressed |
carcinoembryonic antigen (CEA) 362 ng/106 cells/10 days; keratin |
| Tumorigenic |
No |
| Effects |
No, in immunosuppressed mice Yes, in semisolid medium |
| Comments |
Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity.
N-myc, K-ras, N-ras and myb oncogene expression were not detected.
The cells are positive for keratin by immunoperoxidase staining.
The line is positive for expression of c-myc, H-ras, sis and fos oncogenes. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 1 to 2 times per week |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C Atmosphere: air, 100% |
| Isoenzymes |
ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1 |
| Name of Depositor |
A Leibovitz |
| Deposited As |
Homo sapiens |
| References |
Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345
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