| 產(chǎn)品名稱 |
T4 Clone 5 (10-0101, 0062-83) |
| 商品貨號(hào) |
B165817 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against thyroxine (T4, 3,5,3',5'-tetraiodo-L-thyronine) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against thyroxine (T4, 3,5,3',5'-tetraiodo-L-thyronine) |
| Comments |
Animals were immunized with T4 conjugated to chicken gamma globulin. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The extent of cross-reaction with triiodothyronine (T3) is approximately 2%. |
| Complete Growth Medium |
Culture medium: RPMI 1640 with 2 mM L-glutamine adjusted to contain 1.5 g/L
sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 92.5%; DMSO, 7.5% |
| Isotype |
IgG1 |
| Name of Depositor |
Becton, Dickinson and Co. |
| U.S. Patent Number |
|
| References |
Siebert GR, Armstrong J. Monoclonal antibodies recognizing L-thyroxine. US Patent 4,636,478 dated Jan 13 1987
Siebert GR, Armstrong J. Monoclonal antibodies recognizing L-thyroxine. US Patent 4,888,296 dated Dec 19 1989
|
