| 產品名稱 |
TE3 [TE3A] |
| 商品貨號 |
B165844 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an intracellular antigen expressed in cortical, but not medullary, thymic epithelium. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human thymus (cortical thymic epithelium) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human thymus (cortical thymic epithelium) |
| Comments |
Animals were immunized with human thymic stroma. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with an intracellular antigen expressed in cortical, but not medullary, thymic epithelium. All TE3+ cells in the thymus were found to express HLA and Ia antigens, and contain keratin. |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
heat-inactivated fetal bovine serum, 20%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2 |
| Name of Depositor |
BF Haynes |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
McFarland EJ, et al. The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment. J. Immunol. 133: 1241-1249, 1984. PubMed: 6205074
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