| 產品名稱 |
UC3-10A6 |
| 商品貨號 |
B165905 |
| Organism |
Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the murine T cell receptor gamma/delta TCR. The antibody is specific for receptors containing the V gamma 2 segment. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR) |
| Comments |
Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the murine T cell receptor gamma/delta TCR. The antibody is specific for receptors containing the V gamma 2 segment. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG |
| Name of Depositor |
JA Bluestone |
| Deposited As |
hamster, Armenian (B cell); mouse (myeloma) |
| References |
Dent AL, et al. Self-reactive gamma delta T cells are eliminated in the thymus. Nature 343: 714-719, 1990. PubMed: 1968227
|
