| 產(chǎn)品名稱 |
V79-4 |
| 商品貨號(hào) |
B165920 |
| Organism |
Cricetulus griseus, hamster, Chinese |
| Tissue |
lung |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
male |
| Applications |
Used in studies of X-ray induced damage and repair processes. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 22; range = 20 to 23, Pseudodiploid. The rate of higher ploidies was 4%. Twelve to fourteen marker chromosomes were common to most cells. These include 1p-, 4q+, 4p+, t(6,?), 7q+ and seven to eight other small markers., Normal N2 and N3 were paired; N1, N5, N6 and N10 were single. Normal X and Y were absent, but a single Xq- was present in every cell. |
| Derivation |
In 1966, E.H.Y. Chu obtained the line from W. Sinclair, and isolated the V79-4 clone. This cell line was developed by Ford and Yerganian in 1958 from lung tissue of a young male Chinese hamster, and was originally designated Strain V. A culture at passage 7 after cloning was submitted to the ATCC in July 1988. |
| Clinical Data |
male
|
| Comments |
The cells have a high plating efficiency (80%), and a generation time of 12 to 14 hours.
The line was renamed V79 by Elkind in 1958. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
-
Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
-
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:14 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C
Atmosphere: , Air: 95% CO2: 5% |
| Name of Depositor |
EH Chu |
| Deposited As |
Cricetulus griseus |
| Passage History |
A culture at passage 7 after cloning was submitted to the ATCC in July 1988. |
| Year of Origin |
1958 |
| References |
. Molecular cell genetics. New York: Wiley; 1985.
Chu EH, et al. Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro. Genetics 62: 359-377, 1969. PubMed: 5392645
Chu EH, Malling HV. Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc. Natl. Acad. Sci. USA 61: 1306-1312, 1968. PubMed: 5249812
. . Nature 184: 1293-1295, 1959.
Ford DK, Yerganian G. Observations on the chromosomes of Chinese hamster cells in tissue culture. J. Natl. Cancer Inst. 21: 393-425, 1958. PubMed: 13576097
. Molecular cell genetics. New York: Wiley; 1985.
Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260
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