| 產品名稱 |
VD-10 |
| 商品貨號 |
B165922 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a 250000 dalton polypeptide that is part of the saxitoxin binding sodium channel protein in native electroplex membrane. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified saxitoxin binding component of electric eel (E. electricus) electroplex membrane. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against saxitoxin binding component of electroplex membrane |
| Cellular Products |
immunoglobulin; monoclonal antibody; against saxitoxin binding component of electroplex membrane |
| Comments |
Animals were immunized with purified saxitoxin binding component of electric eel (E. electricus) electroplex membrane. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with a 250000 dalton polypeptide that is part of the saxitoxin binding sodium channel protein in native electroplex membrane. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
RPMI 1640 medium, 80%; fetal bovine serum, 20%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
LC Fritz, JP Brockes |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Moore HP, et al. Isolation and characterization of a monoclonal antibody against the saxitoxin-binding component from the electric organ of the eel Electrophorus electricus. Proc. Natl. Acad. Sci. USA 79: 1673-1677, 1982. PubMed: 6280195
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