| 產品名稱 |
vT{2} |
| 商品貨號 |
B165930 |
| Organism |
Mus musculus, mouse |
| Tissue |
liver; stroma |
| Cell Type |
epithelial |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 Cells contain SV40 and CMV viral DNA
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hepatoma |
| Strain |
C57L/J |
| Applications |
The vT{2} cell line expresses the human aryl hydrocarbon receptor nuclear translocator (ARNT) gene The vectors contain cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. The parental cell line c4 (B13NBii1) (ATCC CRL-2717) lacks functional ARNT while its derivative vT{2} (ATCC CRL-2712) possesses a complete transfected ARNT cDNA. Together, they can be used to study ARNT processes and the role of ARNT in vivo. ARNT is directly involved in the regulation of xenobiotic metabolism (including chemical carcinogenesis), hypoxia and differentiation during embryogeneses. The vT{2} cell line was derived by co-transfection of a 6 thioguanine-resistant derivative of c4 (B13NBii1) [ATCC CRL-2717] cell line using the plasmid pSV2gpt and pBM5/NEO-M1-1. M1-1 is a cDNA clone containing the entire human ARNT cDNA sequence. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
The vT{2} cell line was derived by co-transfection of a 6 thioguanine-resistant derivative of c4 (B13NBii1) [ATCC CRL-2717] cell line using the plasmid pSV2gpt and pBM5/NEO-M1-1. M1-1 is a cDNA clone containing the entire human ARNT cDNA sequence. The cells were expanded in G418 to obtain vT{2} (ATCC CRL-2712). The vT{2} cell line expresses the human aryl hydrocarbon receptor nuclear translocator (ARNT) gene The vectors contain cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. ARNT is directly involved in the regulation of xenobiotic metabolism (including chemical carcinogenesis), hypoxia and differentiation during embryogeneses. The parental cell line c4 (B13NBii1) (ATCC CRL-2717) lacks functional ARNT while its derivative vT{2} (ATCC CRL-2712) possesses a complete transfected ARNT cDNA. Together, they can be used to study ARNT processes and the role of ARNT in vivo. |
| Comments |
The vT{2} cell line was derived by co-transfection of a 6 thioguanine-resistant derivative of c4 (B13NBii1) [ATCC CRL-2717] cell line using the plasmid pSV2gpt and pBM5/NEO-M1-1. M1-1 is a cDNA clone containing the entire human ARNT cDNA sequence. The cells were expanded in G418 to obtain vT{2} (ATCC CRL-2712). The vT{2} cell line expresses the human aryl hydrocarbon receptor nuclear translocator (ARNT) gene The vectors contain cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. ARNT is directly involved in the regulation of xenobiotic metabolism (including chemical carcinogenesis), hypoxia and differentiation during embryogeneses. The parental cell line c4 (B13NBii1) (ATCC CRL-2717) lacks functional ARNT while its derivative vT{2} (ATCC CRL-2712) possesses a complete transfected ARNT cDNA. Together, they can be used to study ARNT processes and the role of ARNT in vivo. |
| Complete Growth Medium |
Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine supplemented with 0.4 mg/ml G418, 90%; heat-inactivated fetal bovine serum, 10%
|
| Subculturing |
Protocol: - Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. -
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended Medium Renewal: Every 2 to 3 day |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor |
O Hankinson |
| Deposited As |
mouse |
| References |
Hoffman EC, et al. Cloning of a factor required for activity of the Ah (dioxin) receptor. Science 252: 954-958, 1991. PubMed: 1852076
The vT{2} cell line was derived by co-transfection of a 6 thioguanine-resistant derivative of c4 (B13NBii1) [ATCC CRL-2717] cell line using the plasmid pSV2gpt and pBM5/NEO-M1-1. M1-1 is a cDNA clone containing the entire human ARNT cDNA sequence. The cells were expanded in G418 to obtain vT{2} (ATCC CRL-2712). The vT{2} cell line expresses the human aryl hydrocarbon receptor nuclear translocator (ARNT) gene The vectors contain cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. ARNT is directly involved in the regulation of xenobiotic metabolism (including chemical carcinogenesis), hypoxia and differentiation during embryogeneses. The parental cell line c4 (B13NBii1) (ATCC CRL-2717) lacks functional ARNT while its derivative vT{2} (ATCC CRL-2712) possesses a complete transfected ARNT cDNA. Together, they can be used to study ARNT processes and the role of ARNT in vivo.
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