| 產(chǎn)品名稱 |
XMMCO-791 |
| 商品貨號(hào) |
B165970 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
sarcoma, Carcinoma |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with a tumor associated antigen present in human colorectal carcinoma. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against colorectal carcinoma associated tumor antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against colorectal carcinoma associated tumor antigen |
| Comments |
Animals were immunized with the 791T osteogenic sarcoma cell line (see ATCC CRL-7798). Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody reacts with a tumor associated antigen present in human colorectal carcinoma. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2b |
| Name of Depositor |
Xoma Corporation |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Baldwin RW. Radioimmuno detection of human cancers using anti-tumor monoclonal antibody. US Patent 4,708,862 dated Nov 24 1987
|
