| 產(chǎn)品名稱 |
Y-3P |
| 商品貨號(hào) |
B165984 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k. It cross-reacts with all rat I-A like molecules. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against I-A; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against I-A; cytotoxic |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with spleen cells from (BALB/c X C57BL/6)F1 mice and boosted with spleen cells from BALB/c mice immunized with either BALB.B or C57BL/6 T cell blasts. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k. It cross-reacts with all rat I-A like molecules. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640 with 10 mM HEPES, 95%; fetal bovine serum, 5%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a |
| Name of Depositor |
C Janeway |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Janeway CA Jr., et al. Monoclonal antibodies specific for Ia glycoproteins raised by immunization with activated T cells: possible role of T cellbound Ia antigens as targets of immunoregulatory T cells. J. Immunol. 132: 662-667, 1984. PubMed: 6228596
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