| 產(chǎn)品名稱 |
DMS 114 |
| 商品貨號(hào) |
B167176 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
carcinoma; small cell lung cancer |
| Age |
68 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Derivation |
The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung.
The patient had not received prior therapy. |
| Clinical Data |
68 years
Caucasian
male |
| Antigen Expression |
Leu 7 +; My23 +; CD11b + |
| Receptor Expression |
epidermal growth factor (EGF); complement (CR3) |
| Genes Expressed |
adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) |
| Cellular Products |
adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) |
| Tumorigenic |
Yes |
| Effects |
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Comments |
The patient had not received prior therapy.
The cells express HLA class I and class II antigens.
Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. |
| Complete Growth Medium |
Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 12 D5S818: 12 D7S820: 10,11 THO1: 8,9.3 TPOX: 8,11 vWA: 16,17 |
| Name of Depositor |
OS Pettengill, G Sorenson |
| Deposited As |
Homo sapiens |
| Passage History |
Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. |
| References |
Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631
Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. PubMed: 6249655
|
