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HFBCT78

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編號:B205469

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產品名稱	HFBCT78
商品貨號	B205469
Designations	HFBCT78
GenBank Number	T05242
Species	Homo sapiens, human
Depositors	JC Venter
Vector	Construct size (kb): 3.799999952316284 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ', <- primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, <- promoter: lac, <- primer site: M13 reverse, <- replicon: pMB1 marker(s): ampR, <- Cross references:
Insert	DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PLA2G2A Other gene symbols: PLA2L; PLA2B Gene name: phospholipase A2, group IIA (platelets, synovial fluid) Contains complete coding sequence?: U Chromosome: 1; Localization: 1 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 0.8 Cross references: DNA Seq. Acc.: M86400; EST: EST03131 Nucleotides 1-193 of the insert correspond to nucleotides 2581-2388 of M86400 with 100% identity. DNA Seq. Acc.: T05242; EST: EST03131; Primer: M13-21 Insert lengths(kb): 0.800000011920929 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: phospholipase A2, group IIA (platelets, synovial fluid)(phospholipase A2 polypeptide B (synovial fluid)) [PLA2B]
Insert Size (kb)	0.800
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8. Based on the insert size and the match to the published sequence, probably a partial clone consisting primarily of 3' untranslated region, because the coding sequence of M86400 is nt 85-822.
References	Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

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