Constructed from pPyLT-1 by removing the EcoRI fragment. Stop codon lies in plasmid. Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BamHI/BglI--2.2, 1.8, 0.8, 0.6, 0.3; BamHI/EcoRI--3.8, 2.2. The insert contains a truncated portion of the Large T Antigen. |