Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.
Biosafety Level
N/A
Quality Control Specifications
Subcultures of hiPSCs passaged with Stem Cell Dissociation Reagent maintained pluripotency as determined by expression of pluripotent markers. Detachment of stem cells from tissue culture plastic is also demonstrated.
Unit Definition: One unit releases Folin positive amino acids from casein equivalent to 1 µmole of tyrosine per minute at 37°C and pH 7.5.
A Certificate of Analysis is available upon request.
