Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.9; BamHI--3.8; HindIII--3.8; PstI--2.9, 0.9; KpnI--3.8. The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.03; 0.9; AccI--0.28; BamHI--0.8. Extends from nt 304-1227 of the reported sequence, encoding exons 6-8. Can be used as a probe for the rat tropoelastin gene. |
