Restriction digests of the clone give the following sizes (kb): PstI--6.6; PvuI--6.6; SspI--3.7, 2.9; PvuII--6.2, 0.46; BamHI--4.0, 2.4. Cloning into the HincII, PstI, PvuI, ScaI, or SspI sites inactivates ampR. Cloning into the BalI or NcoI sites inactivates cmlR. Precautions have not been taken to maintain the F' in the JM109 host. The F' is not required to use this plasmid. Shuttle vector. The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - cmlR. |
