Restriction digests of the clone give the following sizes (kb): BamHI--2.6, 1.1; SalI--3.4, 0.4; PvuII--3.4; XbaI--3.5; SmaI--3.7; PstI--2.4, 1.1, 0.4. The open reading frame contains 257 codons, flanked by 51 bp of 5' untranslated region and 259 bp of 3' untranslated region. Encodes complete sequence of DPw2 chain. BamHI site at 3' end is within SV40 sequence (coordinates 0.715-0.710). Insert cut out with BamHI will include approx. 35 bp of SV40 sequence. |
