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pHE-A1 [he-A1]

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編號:B206706

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產品名稱	pHE-A1 [he-A1]
商品貨號	B206706
Designations	pHE-A1 [he-A1]
Species	Homo sapiens, human
Vector	Construct size (kb): 8.100000381469727
Insert	DNA: genomic Insert lengths(kb): 3.900000095367432 Gene product: thyroid hormone receptor, alpha 1 (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 1, formerly ERBA1) [ERBA1] Alleles: A1, A1, A2, A2
Insert Size (kb)	3.900
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 4.2; HindIII--4.8, 2.8; BamHI--8.3. A 2.9 kb EcoRI/HindIII subfragment detects a PvuII RFLP. Insert includes 2.4 kb of unique sequence and a 1.5 kb HindIII/EcoRI fragment containing repetitive sequence. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. The PvuII polymorphism has demonstrated co-dominant segregation in 2 families. Only one allele is present in mouse/human hybrids with one chromosome 17. This is a subclone of lambdahe-A1 (p01239). Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, HinfI, KpnI, PstI, TaqI.
References	Jansson M, et al. Isolation and characterization of multiple human genes homologous to the oncogenes of avian erythroblastosis virus. EMBO J. 2: 561-565, 1983. PubMed: 6313346 Spurr NK, et al. Chromosomal localisation of the human homologues to the oncogenes erbA and B. EMBO J. 3: 159-163, 1984. PubMed: 6323162 Rider SH, et al. RFLP for the human erb-A1 gene. Nucleic Acids Res. 15: 863, 1987. PubMed: 2881264 Douglas JB, et al. A pulsed-field gel electrophoresis map locates the polymorphic probes for ERBA2 and ErbAbeta within 120 kb of each other, confirming that THRB (formerly ERBA2) maps to chromosome 3. Genomics 9: 398-400, 1991. PubMed: 1672299 Barbara Weiffenbach, personal communication

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