Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 0.5, 0.4; BglI--1.54, 1.32, 0.9, 0.04; EcoRI--3.87; EcoRI/XbaI--3.0, 0.87; NcoI--3.44, 0.43. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.36; BglI--0.07, 0.11; NcoI--0.29, 0.72; SacI--0.55, 0.78. Corresponds to nt 1137-2006 of GenBank accession X70811, spanning a region that contains an intron in the genomic sequence. Constructed after amplification from cDNA with these primer/adapters: 5'-TGCGAATTCTGCCTTCAACCCGCTC-3'; 5'-ACTATAGGGTCTAGAGGATCCG-3'. The product was digested with EcoRI + XbaI. Transcription from the SP6 promoter gives the sense strand. |
