Features: marker(s): kanR, spcR, strR replicon: Sa
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--10.5; EcoRI--10.5; PstI--10.5; BglII--10.5; BamHI/EcoRI--8.0, 2.5; BamHI/BglII--6.2, 4.3.
Nonconjugative. Caution: this plasmid is readily lost from the Escherichia coli host. Always grow under antibiotic selection.
A broad host range plasmid vector.
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References
Leemans J, et al. Broad-host-range cloning vectors derived from the W-plasmid Sa. Gene 19: 361-364, 1982. PubMed: 6295888
Shipped
freeze-dried
Shipping Information
Shipped: ?Freeze dried E. coli containing the plasmid
