Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2)
Gene product: major histocompatibility complex, class II, DQ alpha 1 [HLA-DQA]
Insert Size (kb)
1.300
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): PstI/XhoI--1.85, 1.35, 1.2; PstI--2.8, 1.2; XhoI--3.9; HindIII--3.9; SalI--3.5 + others; BamHI-- 3.2, 1.2.
The insert contains internal ApaI and StuI sites about 0.1 and 0.79 kb from the 5' BamHI.
The insert follows a 5' GC tail of 15 bp and contains 50 bp of 5' untranslated sequence.
References
Sekaly RP, et al. Cell surface expression of class II histocompatibility antigens occurs in the absence of the invariant chain. J. Exp. Med. 164: 1490-1504, 1986. PubMed: 2430042
