Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2)
Gene product: major histocompatibility complex, class II, DR alpha(major histocompatibility locus, class II, DR alpha) [HLA-DRA]
Insert Size (kb)
1.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI/XhoI--1.9, 1.2, 0.7 doublet; PstI--2.5, 1.2, 0.7; XhoI--4.2; HindIII--4.2; SalI--3.7 + others; BamHI--3.0, 1.3.
The insert contains internal SacI, BclI, PstI, MstI and NcoI sites about 0.06, 0.17, 0.65, 0.8 and 1.15 kb from 5' BamHI.
The insert follows a 5' GC tail of 16 bp and contains 73 bp of 5' untranslated sequence.
References
Sekaly RP, et al. Cell surface expression of class II histocompatibility antigens occurs in the absence of the invariant chain. J. Exp. Med. 164: 1490-1504, 1986. PubMed: 2430042
