vector permitting production of single-stranded DNA
Comments
The plasmid is lost at 42C if grown without antibiotic. The host Escherichia coli strain must express gene 2 of filamentous bacteriophages.
Vector with bacteriophage fd replication system.
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References
Geider K, et al. A plasmid cloning system utilizing replication and packaging functions of the filamentous bacteriophage fd. Gene 33: 341-349, 1985. PubMed: 2989095
